个人信息

博士生导师
研究员

Email: lixuan@cemps.ac.cn
个人网页: https://people.ucas.ac.cn/~0617

研究方向

基因组学和合成生物学

李轩

个人简介

1988年 获北京大学生物系学士学位
1996年 获美国 St Louis University 生物化学博士学位
1997-2000年 美国 Duke University 博士后
2009年至今任中国科学院上海植物生理生态所研究员。2010年入选上海市浦江人才计划。前后承担了包括国家重点基础研究发展项目(973)、国家高技术研究发展计划(863)、国家科技重大专项、国家重点研发计划、国家自然科学基金、中科院先导A等项目/课题。现担任上海生物信息学会理事、中国医药生物技术协会生物医学信息分会常务委员。

研究工作

研究组基于分子生物学、基因组学实验技术、和人工智能深度学习、生物信息学方法,围绕合成生物学的重大问题,开展以下方向的研究:
1)表观合成生物学:RNA编辑机制研究、RNA编辑人工机器构建、和RNA基因治疗技术。
2)天然产物发现和功能表征:中药药用植物/深海微生物的 (宏)基因组和 (宏)代谢组分析方法技术和功能表征研究、遗传调控网络解析。

主要成果

1、表观合成生物学:RNA编辑机制研究、RNA编辑人工机器构建、和RNA基因治疗技术。
1)实现CRISPR-Cas13a介导的精确定点RNA编辑的人工机器
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gky433/5025895
2)通过分割APOBEC3A双重酶活性实现构建特异性RNA碱基编辑酶
https://pubs.acs.org/doi/10.1021/acssynbio.0c00627
3)利用RNA编辑策略实现对逆转录转座子的编辑干预
https://www.nature.com/articles/s41421-020-0164-0
4)利用CRISPR-Cas13b抑制寨卡病毒的复制和感染
https://www.sciencedirect.com/science/article/pii/S0168170222000351
5)RNA编辑表观遗传位点的功能和系统进化
http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006191

2、天然产物发现和功能表征研究:中药药用植物/深海微生物的 (宏)基因组和 (宏)代谢组分析方法技术和功能表征、遗传调控网络解析。
1)应用深度代谢组学分析方法,完成水稻群体材料的代谢组分析,并把代谢产物多样性增大了一个数量级;利用共表达网络建立黄酮类的代谢网络,发现新修饰结构的黄酮类代谢产物。
https://www.sciencedirect.com/science/article/pii/S1672022921000309
2)对传统中药(TCM)的活性分子进行高通量细胞表征组实验研究,通过与CMAP模式分析比对解析中药活性分子的功能,并应用于102种中药活性分子的研究,解析了多种中药活性成分的作用机制和多组分间的协同作用,为传统中药的研究提供了新思路。
https://www.nature.com/articles/s41598-017-00535-8/
3)豆科药用植物黄芪的代谢组和转录组的整合分析研究。通过深度代谢组分析,发现黄芪中未知存在的一系列化合物,包括黄酮类、萜类化合物、生物碱类、和苯丙素类。重构了黄芪完整代谢profile和重要药用成分的合成途径。
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07005-y
4)构建了国际上第一个植物分泌蛋白数据库和代谢组数据库。提供植物代谢谱数据及搜索和鉴定代谢物的算法工具。
https://www.sciencedirect.com/science/article/pii/S1672022921000309
https://academic.oup.com/nar/article/41/D1/D1192/1056857

    Qin H?, Ou L?, Gao J, Chen L, Wang JW*, Hao P*, Li X*. DENA: training an authentic neural network model using Nanopore sequencing data of Arabidopsis transcripts for detection and quantification of N6-methyladenosine on RNA. Genome Biol. 2022; 23(1):25.

    Chen P, Chen M, Chen Y, Jing X, Zhang N, Zhou X, Li X*, Long G*, Hao P*. Targeted inhibition of Zika virus infection in human cells by CRISPR-Cas13b. Virus Res. 2022. doi: 10.1016/j.virusres.2022.198707.

    Chen P, Zhou H, Huang Y, Xie Z, Zhang M, Wei Y, Li J, Ma Y, Luo M, Ding W, Cao J, Jiang T, Nan P*, Fang J*, Li X*. Revealing the full biosphere structure and versatile metabolic functions in the deepest ocean sediment of the Challenger Deep. Genome Biol. 2021; 22(1):207.

    Zhou H, Chen P, Zhang M, Chen J*, Fang J*, Li X*. Revealing the Viral Community in the Hadal Sediment of the New Britain Trench. Genes (Basel). 2021; 12(7):990.

    Tang G, Xie B, Hong X, Qin H, Wang J, Huang H, Hao P*, Li X*. Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates. ACS Synth Biol. 2021;10(5): 1106-1115.

    Li X, Zhou H, Xiao N, Wu X, Shan Y, Chen L, Wang C, Wang Z, Huang J*, Li A*, Li X*. Expanding the Coverage of the Metabolic Landscape in Cultivated Rice with Integrated Computational Approaches. Genomics Proteomics Bioinformatics 2021; S1672-0229(21)00030-9.

    Wu X, Li X, Wang W, Shan Y, Wang C, Zhu M, La Q, Zhong Y, Xu Y*, Nan P*, Li X*. Integrated metabolomics and transcriptomics study of traditional herb Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao reveals global metabolic profile and novel phytochemical ingredients. BMC Genomics 2020; 21(Suppl 10):697.

    Zhang N, Jing X, Liu Y, Chen M, Zhu X, Jiang J, Wang H, Li X*, Hao P*. Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a. Cell Discovery 2020; 6:30.

    Jing X#, Xie B#, Chen L, Zhang N, Jiang Y, Qin H, Wang H, Hao P*, Yang S*, Li X*. Implementation of the CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing. Nucleic Acids Res. 2018; 46(15):e90.

    Chen P, Jing X, Ren J, Cao H, Hao P*, Li X*. Modeling BioNano optical data and simulation study of genome map assembly. Bioinformatics 2018; 34(23):3966-3974.

    Shen F#, Wu X#, Shi L, Zhang H, Chen Y, Qi X, Wang Z*, and Li X*. Transcriptomic and metabolic flux analyses reveal shift of metabolic patterns during rice grain development. BMC Systems Biology 2018; 12(Suppl 4):47.

    Cao Y, Cao R, Huang Y, Zhou H, Liu Y, Li X*, Zhong W*, Hao P*. A comprehensive study on cellular RNA editing activity in response to infections with different subtypes of influenza A viruses. BMC Genomics 2018; 19(Suppl 1):925.

    Chen P#, Jing X#, Liao B, Zhu Y, Xu J, Liu R, Zhao Y, and Li X*. BioNano map resource for Oryza sativa ssp. japonica and indica and its application in rice genome sequence correction and gap filling. Molecular Plant 2017; 10, 895–898.

    Zhu Y#, Chen L#, Zhang C, Hao P, Jing X* and Li X*. Global transcriptome analysis reveals extensive gene remodeling, alternative splicing and differential transcription profiles in nonseed vascular plant Selaginella moellendorffii. BMC Genomics 2017; 18(Suppl 1):1042.

    Lv C#, Wu X#, Wang X, Su J, Zeng H, Zhao J, Lin S, Liu R, Li H*, Li X*, Zhang W*. The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs. Scientific Reports 2017; 7:352.

    Yu Y, Zhou H, Kong Y, Pan B, Chen L,Wang H, Hao P* and Li X*. The landscape of A-to-I RNA editome is shaped by both positive and purifying selection. PLoS Genetics 2016; 12(7): e1006191.

    Kong Y, Zhou H, Yu Y, Chen L, Hao P*, Li X*. The evolutionary landscape of intergenic trans-splicing events in insects. Nature Communications 2015; 6:8734.

    Pan B, Sheng J, Sun W, Zhao Y, Hao P*, Li X*. OrysPSSP: a comparative platform for small secreted proteins from rice and other plants. Nucleic Acids Res. 2013; 41(D1):D1192-8.